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cell / tissure disruption methods 예비레포트 입니다. 꼼꼼하게 작성한 레포트로 실험제목/ 목표 / 방법/ 결과 / 고찰 / 출처 자세하게 기재되어 있습니다

저작시기 2017.11 |등록일 2018.01.12 MS 워드 (docx) | 3페이지 | 가격 500원

목차

1. Cell or tissue disruption methods /lysis
2. Protein purification
3. Protein concentration
4. dialysis

본문내용

Cell or tissue disruption methods /lysis
Many cell lysis methods have been developed to obtain the best possible yield and purity for different species of organisms, sample types (cells or tissue), and target molecule or subcellular structure. Cell lysis methods can be divided into two main categories, reagent-based and physical disruption.

In recent years, detergent-based lysis methods have become the norm. Through empirical testing by trial and error, different detergent-based solutions composed of particular types and concentrations of detergents, buffers, salts and reducing agents have been developed to provide the best possible results for particular species and types of cells.

참고 자료

https://en.wikipedia.org/wiki/Dialysis
http://www.merckmillipore.com/KR/ko/life-science-research/protein-sample-preparation/protein-purification/Gdib.qB.6CIAAAE_ztx3.Mmz,nav
https://www.thermofisher.com/kr/ko/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/pierce-protein-methods/overview-cell-lysis-and-protein-extraction.html
https://en.wikipedia.org/wiki/Ammonium_sulfate_precipitation
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